Microbial Reproduction and Growth
Reproduction patterns. During their growth cycles, microorganisms undergo reproduction many times, causing the numbers in the population to increase dramatically.
In fungi, unicellular algae, and protozoa, reproduction involves a duplication of the nucleus through the asexual process of mitosis and a splitting of the cell in cytokinesis. Reproduction can also occur by a sexual process in which haploid nuclei unite to form a diploid cell having two sets of chromosomes. Various changes then follow to yield a sexually produced offspring. Sexual reproduction has the advantage of mixing chromosomes to obtain genetic variations not possible with asexual reproduction. However, fewer individuals normally result from sexual reproduction than from asexual reproduction. More details on these methods are provided in the chapters on fungi and protozoa.
Bacteria reproduce by the asexual process of binary fission. In this process, the chromosomal DNA duplicates, after which the bacterial membrane and cell wall grow inward to meet one another and divide the cell in two. The two cells separate and the process is complete.
One of the remarkable attributes of bacteria is the relatively short generation time, the time required for a microbial population to double in numbers. The generation time varies among bacteria and often ranges between 30 minutes and three hours. Certain bacteria have very brief generation times. Escherichia coli, for example, has a generation time of about 20 minutes when it is dividing under optimal conditions.
The growth curve. The growth of a bacterial population can be expressed in various phases of a growth curve. The logarithms of the actual numbers in the population are plotted in the growth curve along the side axis, and the time is plotted at the base. Four phases of growth are recognized in the growth curve.
In the first phase, called the lag phase, the population remains at the same number as the bacteria become accustomed to their new environment. Metabolic activity is taking place, and new cells are being produced to offset those that are dying.
In the logarithmic phase, or log phase, bacterial growth occurs at its optimal level and the population doubles rapidly. This phase is represented by a straight line, and the population is at its metabolic peak. Research experiments are often performed at this time.
During the next phase, the stationary phase, the reproduction of bacterial cells is offset by their death, and the population reaches a plateau. The reasons for bacterial death include the accumulation of waste, the lack of nutrients, and the unfavorable environmental conditions that may have developed. If the conditions are not altered, the population will enter its decline, or death phase (Figure 1 ). The bacteria die off rapidly, the curve turns downward, and the last cell in the population soon dies.