Bioengineering questions and answers in October 2022

Imagine you have a good preparation of genomic DNA. You want to see if you could use that as a template for dideoxy sequencing. You make the oligonucleotide correctly to be complementary to one specif...
Think of one way (experimentally or deduced from other available prior information) that you can find out whether an STS contains repeated sequence before you use it to probe thousands of BAC clones. ...
In a complete mapping experiment there would be thousands of BACs and each would be tested for thousands of STS markers. How would you extend your map to the left or the right of the contig you got in...
A centrifuge dewaters digested sludge from an anaerobic digester. Pertinent performance data are; raw sludge feed rate to the anaerobic digester = 60,000 lb/day on a dry solids basis, digester suspend...
Generic Pharma LLC. Is also interested in exploring a lipid nanoparticle loaded tablet (customers are more used to tablets) as an alternative to an oral emulsion. Knowing that an addition of mannitol ...