Kino The young Indian pearl diver who finds the magnificent pearl and whose life is partially destroyed by this pearl.
Juana Kino's wife and faithful partner in eking out a meager living; she is obedient and devoted to her family.
Coyotito Coyotito is Kino and Juana's infant son; he is bitten by a scorpion and recovers miraculously only to be later killed by a bullet, a bullet intended for Kino.
Juan Tomás Kino's brother, advisor, and his only protector when Kino is hunted for murder.
Apolonia Juan's fat wife, who has no real significance in the story.
The Doctor A thoroughly heartless, self-seeking man whose love of money is displayed when he refuses to help Coyotito because Kino cannot pay him his fee.
The Priest The discovery of the pearl is said to "put a thoughtful look in his eyes and a memory of certain repairs necessary to the church."
The Pearl Buyers They are unnamed in the story, and as a group, they represent the exploitation and hypocrisy that the Indians encounter.