How has DNA matching really made big difference in finding out who committed a crime?

The use of DNA probes and the development of retrieval techniques have made it possible to match DNA molecules to one another for identification purposes. This process has been used in a forensic procedure called DNA fingerprinting.

The use of DNA fingerprinting depends upon the presence of repeating base sequences that exist in the human genome. The repeating sequences are called restriction fragment length polymorphisms (RFLPs). As the pattern of RFLPs is unique for every individual, it can be used as a molecular fingerprint.

To perform DNA fingerprinting, DNA is obtained from an individual's blood cells, hair fibers, skin fragments, or other tissue. The DNA is extracted from the cells and digested with enzymes. The resulting fragments are separated by a process called electrophoresis. These separated DNA fragments are tested for characteristic RFLPs using DNA probes.

A statistical evaluation enables the forensic pathologist to compare a suspect's DNA with the DNA recovered at a crime scene and to assert with a degree of certainty (usually 99 percent) that the suspect was at the crime scene.